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. 2021 Feb 24;12:620433. doi: 10.3389/fphar.2021.620433

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Copyright © 2021 Xiong, Chang, Hao, Zhang, Yang, Wang, Liu, Wang, Mu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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M2 macrophages are the major source of MMT in the contralateral kidneys of long term-UUO rats. (A) immunofluorescent multi-staining of kidney sections with antibodies against collagen I (blue), macrophage marker F4/80 (green), and α-SMA (red). (B) fluorescent co-staining of α-SMA (red) with M2 macrophage marker CD206 (green, upper panel) or M1 macrophage marker iNOS (green, lower panel), nuclei were stained with DAPI in blue. Images are representative for n = 12 rats each group. Quantification data are presented as mean ± SEM, n = 6. *p < 0.05 vs. the sham, #p < 0.05 vs. the UUO.