TABLE 1.

ZIKV cDNA fragments spanning the whole viral genome used in viral template assembly.

cDNA fragment (genomic position)	Initial cloning vector	pCC1-MCS-Z1Z2 (genomic position)	pCC1-Z3Z4 (genomic position)	Template (genomic position)	Cloning sites
Z1 (1–2,047)	pUC57	1–2,001	–	1–2,001	NotI – KpnI
Z3 (1,653–4,793)	pCC1-4K	–	2,002–4,776	2,002–4,776	KpnI – BamHI
Z4 (4,581–8,855)	pUC57	–	4,777–8,843	4,777–8,843	BamHI – MluI
Z2 (8,844–10,807)	pUC57	8,844–10,807	–	8,844–10,807	MluI – XhoI