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. 2021 Mar 9;4:312. doi: 10.1038/s42003-021-01825-8

Fig. 4. Mutation of NOTCH1/2 enhances restoration of PTEN expression in response to EZH2 inhibition.

Fig. 4

Inhibition of EZH2 by UNC1999 was performed for 7 days and 11 days and transcript levels of PTEN were measured by qRT-PCR in (a) HCC-1187, (b) HCC-1954, (c) HCC-2218, (d) MDA-MB-231, (e) HCC-1143, and (f) MDA-MB-175-VII. Error bars: mean ± s.d., triplicate measurements. Significance from Ctrl: one-way ANOVA, Sidak’s correction. g Table shows the NOTCH1/NOTCH2 mutation status of breast cancer cell lines32,40. h Boxplots of NOTCH1 and PTEN RNA and protein levels in EZH2-high (RNA-seq z-score > 1) and EZH2-low (RNA-seq z-score < 1) breast cancer cases, measured by RNA-seq and RPPA, respectively34. Number of cases indicated for each group (total n = 816). (****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05).