Fig. 7. TIGIT⁺ memory B-cell-modified DCs suppress the induction of IL-21- and IL-4-producing T cell response, while promoting the induction of IL-10-producing CD4⁺ T cell response.

a–d Representative FACS plots (left panels) and summarized data (right panels) showing the induction of CXCR5⁺ICOS⁺ T cell response (a), as well as IL-21- (b), IL-4- (c), and IL-10-expressing (d) CD4⁺ T cell response elicited by monocyte-derived dendritic cells (MDDCs) modified by TIGIT⁺ memory B cells. TIGIT⁺ memory B cells or TIGIT⁻ B cells were cocultured with MDDCs for 2 days in the presence of 100 ng/mL LPS before coculturing them for 7 days with FACS-sorted naive CD4⁺ T cells (CD4⁺CD45RA⁺CD45RO⁻CCR7⁺). PMA, ionomycin, brefeldin A, and monensin cocktails were added 5 h before intracellular staining of indicated cytokines. Isotype antibody staining was performed using mixture of CD4⁺ T cells cultured in three different conditions. Cells were gated based on isotype antibody staining. Data from four independent experiments performed with cells from different healthy individuals (n = 4). Error bars are mean ± SD. P values were determined with one-way ANOVA with Holm–Sidak’s multiple comparison test (a–d).