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. 2021 Mar 9;12:1534. doi: 10.1038/s41467-021-21413-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a, b Representative FACS plots and summarized data for the frequency of CD19⁺CD24^hiCD27⁺ B10-like Bregs (a), and P2 MZ-like and P4 memory B cells (b) in the blood of donor-specific antibody (DSA)⁺ (n = 12) and DSA⁻ (n = 19) liver transplant patients and healthy controls (n = 9). Dots represent individual patients. c, d Scatter plots showing the association of the frequency of B10-like, P2 MZ-like, and P4 memory B cells with CD4⁺CD25⁺CD127^lo Tregs (c) and with CXCR5⁺ICOS⁺ TFH cells (d) in liver allograft recipients. e, f Scatter plots showing the association of the frequency of P4 memory (e) and TIGIT⁺ memory B cells (f) with that of CD4⁺CD25⁺CD127^lo Tregs in renal allograft recipients. g, h Scatter plots showing the association of the frequency of P4 memory B cells (g) and TIGIT⁺ memory B cells (h) with that of CD4⁺CXCR5⁺ICOS⁺ TFH in renal allograft recipients. Error bars indicate mean ± SD. P values were determined with one-way ANOVA with Dunnett’s multiple comparison test (a, b). R² and P values were calculated using Pearson correlation tests (c–h).