Figure 6.

Induction of nuclear localization of the aryl hydrocarbon receptor (AhR) by cyproterone acetate (CPA) in mouse cells. Hepa-1c1c7 cells were treated with CPA (30, 60 and 90 μM) and β-NF (10 μM) for 2 h, and then cells were fixed with 4% formaldehyde, and nuclei were stained with Hoechst 33342 (5 μg/ml). Expression of the AhR protein was probed using an antibody against the AhR, as revealed by the fluorescence of a rabbit polyclonal secondary antibody to goat IgG-H&L (DyLight 488). Fluorescence emitted by DyLight 488 was viewed using a fluorescence microscope, equipped with optical filters at excitation/emission wavelengths of 493/518 nm. Fluorescence emitted by Hoechst 33342 was viewed using a fluorescence microscope, equipped with optical filters at excitation/emission wavelengths of 346/460 nm. Fluorescence images for the AhR and nucleus were merged.