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. 2021 Mar 10;8:59. doi: 10.1038/s41438-021-00491-5

Fig. 3. Acceleration of seed senescence in PbEIL1-overexpressing tomato.

Fig. 3

a PbACS and PbACO expression patterns at different stages in seeded pear cultivars and ‘1913’. b Tissue-specific and spatiotemporal expression of PbEIL1. c Identification of positive transgenic plants by GUS staining. The black arrows indicate the longitudinal sections of the ovules. The staining revealed the expression of PbEIL1 under the control of the PeFBP7 promoter in the ovule integument. d Seed development of 20-day-old fruits. The black arrows point to areas of significant seed senescence. e Paraffin sections of developing integument cells stained with Alcian blue and nuclear fast red. The black arrows indicate regions where the structures of transgenic and WT cells were significantly different. f Observation of seed death by trypan blue staining. The inner integuments of transgenic seeds were obviously stained (red arrows). In (a) and (b), the expression levels of each gene in ‘Starkrimson’ at 0 DAP and the expression of PbEIL1 in petals were normalized to 1.0. The data are mean ± SDs of three biological replicates. Different letters between bars indicate significant differences at P < 0.05 (Duncan’s multiple range test)