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. 2021 Mar 9;11:5479. doi: 10.1038/s41598-021-84955-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA expression of ECMs in 3T3-L1 3D organoids under several conditions. At Day 7, the 3T3-L1 3D organoids of preadipocytes (DIF−) and their adipogenic differentiation (DIF+) without or with 10 µM Ripasudil (Rip) or Y27632 were subjected to a qPCR analysis to estimate the mRNA expression of ECMs (Col1: collagen 1, Col4: collagen 4, Col6: collagen 6, Fn: fibronectin). All experiments were performed in duplicate using fresh preparations, each of which consisted of 16 organoids. Data are presented as arithmetic means ± standard error of the mean (SEM). **P < 0.01, ***P < 0.005, and other pairs were not statistically significant (ANOVA followed by a Tukey’s multiple comparison test).