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. 2021 Feb 24;10:623048. doi: 10.3389/fonc.2020.623048

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Copyright © 2021 Zhong, Qiu, Liu, Yang, Gu, Wang, Chen, Liu, Miao and Zhuang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in the expression of tumor necrosis factor-induced protein-8 (TIPE) affect cell migration. (A) After 12, 24, and 36 h incubation, in vitro wound healing assays with TIPE or shTIPE transfected HCT116 cells were performed to evaluate cell migration. (B) Histograms indicated the size of the wound healing area, and representative statistical results are shown. (C) Based on a Transwell assay, cell migration was significantly enhanced after transfection with TIPE in HCT116 cells but decreased after transfection with shTIPE. Quantitative analysis of three independent experiments is shown in (D); ns, not significant, **p< 0.01, ***p< 0.001.