Figure 3.

Parkin deregulation is involved in the alteration of mitochondrial morphology in PC12 cells treated with α-Syn. (A) Epifluorescence images of mitochondria labeled by transient transfection of pRA306 roGFP in stable pcDNA and pcDNA-Parkin PC12 cells treated with α-Syn for 24 h at a concentration of 5 μM. Scale bar: 10 μm. Quantitative image analysis of (B) mitochondrial branching (form factor) and (C) length (aspect ratio) of pcDNA and pcDNA-Parkin PC12 cells treated with α-Syn. Data represent the mean value ± SD for a representative of three experiments with four fields per experiment (n = 12, different colors represent independent experiments). **p < 0.01, ***p < 0.001 compared to pcDNA control cells; ^#p < 0.05 compared to pcDNA cells treated with α-Syn, using two-way ANOVA followed by Bonferroni post hoc test.