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. 2021 Feb 24;11:572585. doi: 10.3389/fonc.2021.572585

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Copyright © 2021 Jin, Hu, Yan, Jin, Pan, Li, Peng and Cao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Microarray results indicating that HDAC2 expression in MHCC97H cells decreased significantly after irradiation. (B) HDAC1, HDAC2, and HDAC3 activity in tumor (n = 16) and non-tumor (n = 16) tissues. (C) The activity of HDAC2 in the HCC cells Huh7, HepG2, Hep3B, BEL-7402, MHCC97H, and MHCC97L and the human normal liver cells L02. *Compared with the L02 group. (D) The activity of HDAC2 in HepG2 and MHCC97H cells treated with different doses of irradiation. * Compared with the HepG2 and MHCC97H groups. (E, F) The mRNA expression of MIR22HG and miR-22-5p in HepG2 and MHCC97H cells treated with different doses of irradiation (1, 2, 4, and 6 Gy). *Compared with HepG2 and MHCC97H groups. (G) Western blot analysis of histone H3K4ac, H3K9ac, H3K23ac, H3k27ac, and H3 in HepG2 and MHCC97H cells. The treatments were irradiation of 4 Gy alone, irradiation of 4 Gy + histone acetyltransferase inhibitor C646, irradiation of 4 Gy + histone deacetylation inhibitor Santacruzamate A. (H, I) Expression of MIR22HG and miR-22-5p in HepG2 and MHCC97H cells. The treatments were irradiation 4 Gy alone, irradiation of 4 Gy + histone acetyltransferase inhibitor C646, irradiation of 4Gy + histone deacetylation inhibitor Santacruzamate A. * Compared with the NC group. (J) ChIP-PCR showing the amplified signal of the MIR22HG promoter region. The treatments were irradiation of 4 Gy alone, irradiation of 4 Gy + histone acetyltransferase inhibitor C646, and irradiation of 4 Gy + histone deacetylation inhibitor Santacruzamate A. Results showed that both irradiation and drug intervention in HDAC2 can alter amplified signals of the MIR22HG promoter region. Mean ± SD (n = 3 independent experiments). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. San A, Santacruzamate A.