Figure 4.

Bioinformatics analysis identifies dominant signatures of immune–mediated biological processes. Supervised gene ontology (GO) annotation analysis of complement components in hepatocellular carcinoma (HCC) was performed via ToppGene Suite portal (http://toppgene.cchmc.org) for in-silico enrichment of biological processes with a threshold False Discovery Rate (FDR) corrected P value of <0.05. ToppGene Suite is a freely available online tool used for functional enrichment, prioritization of candidate genes using transcriptome, ontology, phenotype, proteome, and functional annotations. GO: biological processes identified by ToppGene were further subjected to functional enrichment using CIMminer (https://discover.nci.nih.gov/cimminer/home.do). Red areas depicted in the heatmap show closely related biological processes linked to immunity that are shared by a major group of complement molecules shown on the horizontal axis.