Figure 8.

Anti-tumor efficacy of MSLN-CAR-T cells against different PDX models in vivo

(A) The result of hematoxylin and eosin (H&E) and antibody against MSLN. (B and G) Schema of the experimental events and nodes. After the establishment of the PDX model, the mice were randomly divided into two groups (PDX-COL0092 n = 5, PDX-GAST0021 n = 4), CAR-T group and NC-T group. (C, D, H, and I) The tumor size (C and H) and mice weight (D and I) variated with MSLN-CAR-T cells and control T cells injection during the observed time. (E) Kaplan-Meier curves for survival of the PDX-COL0092 models are shown. (F) At the end of the experiment in the PDX-COL0092 group, the MSLN-CAR gene copies in the tumor in the experimental group that still had the tumor were detected by qPCR, and representative results from one of three experiments are shown (mean ± SEM; p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).