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. 2021 Feb 28;41:101921. doi: 10.1016/j.redox.2021.101921

Fig. 1.

Fig. 1

Characteristics of iPSCs derived from a MELAS patient. (A) The sequence at the MELAS mutation site of the tRNA gene in mtDNA. The mutated MTTL1 gene that encodes for tRNALeu (UUR) contained a substitution of A to G at mtDNA position 3243 (A3243G). Forward WT and Mut primers, as well as the reverse primer for qPCR analysis, are shown. (B) Staining for the pluripotency markers (OCT4, NANOG, TRA1-60, green; alkaline phosphatase activity, red) in MELAS (A24#1–3) iPSC lines. (C) Copy numbers of WT and Mut mtDNA in the control (454E2) and MELAS (A24#1–3) iPSCs were quantified by qPCR and normalized to nuclear DNA (FBXO15) levels (4 replicates). *P < 0.05: comparison of WT mtDNA levels between control (454E2) and MELAS (A24#1–3) iPSCs; #P < 0.05: comparison of Mut mtDNA levels between control (454E2) and MELAS (A24#1–3) iPSCs. (D) Changes in the number of the control (454E2) and MELAS (A24#1–3) iPSCs during 3 days of maintenance in culture (n = 3; *P < 0.05). (E) Lack of changes in the mitochondrial area in electron microscopy images (n = 30–42). WT, wild-type; Mut, mutant. Scale bar, 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)