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. 2021 Mar 1;23(1):21–31. doi: 10.22074/cellj.2021.7010

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The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.

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Fig.1 — long non-coding RNAs (lncRNAs) plasmacytoma variant translocation 1(PVT1) was up-regulated in non-small cell lung cancer (NSCLC) tissues and cells, promoting NSCLC cell invasion and migration. A. quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis for the expression of PVT1 in the NSCLC and adjacent normal tissues (n=30). B. qRT-PCR analysis for the expression of PVT1 in five NSCLC cell lines compared to the normal lung epithelial cells, BEAS-2B. C. qRT-PCR analysis for the expression of PVT1 in A549 and H1299 cells transfected with three siRNAs of PVT1 (si-PVT1-1, siPVT1-2 and si-PVT1-3) or the negative control (si-NC) as well as pcDNA-PVT1 overexpression vector (PVT1) and pcDNA vector (vector). D. Colony formation assays were used to assess proliferation of A549 and H1299 cells after PVT1 knockdown and PVT1 overexpression. E. Matrigel-free transwell assay for cell migration and F. Matrigel transwell assay for cell invasion were confirmed in A549 and H1299 cells after PVT1 knockdown by siRNAs transfection and PVT1 overexpression. Data are mean ± SD of three independent experiments. *; P<0.05, **; P<0.01, ***; P<0.001 compared to si-NC group, #; P<0.05, and ##; P<0.01; P<0.01 compared to the vector group. PVT1 knockdown inhibited expression of yes-associated protein 1 (YAP1) and NOTCH1 signaling activation.