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. 2021 Mar 1;23(1):21–31. doi: 10.22074/cellj.2021.7010

Fig.2.

Fig.2

Plasmacytoma variant translocation 1(PVT1) knockdown inhibited yes-associated protein 1 (YAP1) expression and Notch1 signaling activation. Western blot analysis for protein levels of LAST2, p-YAP1, t-YAP1, TEAD and Notch1 in the NSCLC and adjacent normal tissues. B. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis for mRNA expression levels of LAST2, YAP1, TEAD and Notch1in A549 and H1299 cells after PVT1 knockdown using the treatment with si-PVT1 or si-NC. C. Western blot analysis for protein levels of LAST2, p-YAP1/t-YAP1, TEAD and Notch1 in A549 and H1299 cells after PVT1 knockdown by treatment with si-lncPVT1 or siRNA negative control (si-NC). D. qRT-PCR analysis for the expression of microRNA-497(miR-497) in A549 and H1299 cells after PVT1 knockdown or overexpression. E. MSP analysis for the methylation level of miR-497 promoter. Data are mean ± SD of three independent experiments. *; P<0.05, **; P<0.01 compared to si-NC group; ##; P<0.01 compared to vector group, p-YAP1; Phosphorylated YAP1 protein, t-YAP1; Total YAP1 protein, si-NC; siRNA negative control, si-PVT1; PVT1 siRNA for knockdown, Vector; Empty pcDNA vector for negative control of overexpression, A549 and H1299; Two of human lung carcinoma cell lines, MSP; Methylation-specific polymerase chain reaction, LAST2; Large tumor suppressor kinase 2 protein, and SD; Standard deviation.