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. 2021 Mar 1;23(1):51–60. doi: 10.22074/cellj.2021.7080

Fig.1.

Fig.1

The high-glucose treatment induced the expression of miR-200b and impaired angiogenesis. A. Quantification of miR-200b by realtime PCR in Human umbilical vein endothelial cell (HUVECs) grown in normal glucose (NG) or high-glucose (HG) conditions for 12, 24, and 48 hours. U6 was used as an internal control for normalization. B. Representative images of HUVECs under different conditions during the in vitro angiogenesis assay. C. Quantification of secreted Vascular Endothelial Growth Factor (VEGF) from HUVECs, as determined by enzyme-linked immunosorbent assay (ELISA) after the indicated treatment. (N = 3). *; P<0.05, **; P<0.01, and H; Hour.

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