Fig.2.

miR-200b affected the angiogenesis ability of Human umbilical vein endothelial cell (HUVECs). The HUVECs cells were transfected with NC mimic, miR200b mimics, NC inhibitor, miR-200b inhibitor, and miR-200b transfection efficiency were analyzed by A. IF imaging and B. Real-time PCR. C. Quantification of HUVEC viability after the indicated treatment, as determined by CCK-8 assays. D. Representative images of HUVECs after the indicated treatments during the wound healing assay. E. Typical images and quantification of HUVECs with different treatments during the migration assay. F. Representative images of HUVECs under different conditions during the in vitro angiogenesis assay. (N = 3). NG; Normal glucose, HG; high-glucose, *; P<0.05, and **; P<0.01.