FIGURE 1.

Dosing schedule for the second in vivo study. The left ventricle and femoral artery were catheterized in normotensive male Sprague-Dawley rats. Subsequently, the animals were randomly chosen to receive either three doses of saline, CMF-019 or [Pyr¹]apelin-13, before a fourth dose of 50 nmol [Pyr¹]apelin-13 was administered regardless of the previous doses administered. Doses were administered at 10 mins intervals or when a stable baseline was reached after which the animal was terminated by exsanguination under high flow isoflurane.