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. 2021 Jan 21;11:588669. doi: 10.3389/fphar.2020.588669

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Copyright © 2021 Read, Nyimanu, Yang, Kuc, Williams, Fitzpatrick, Foster, Glen, Maguire and Davenport.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative contour plot flow data of human PAECs induced to apoptosis with TNFα/CHX and rescued with either rhVEGF or CMF-019 at 1 µM. Cells were incubated in EGM-2 with 10% FBS (control); EGM-2 with 2% FBS and TNFα/CHX (TNFα/CHX) treatment for 5hrs to induce apoptosis before rhVEGF (10 ng/ml) or CMF-019 (1–10 μM) was added for a further 18 h. PI staining is displayed on the x-axis and annexin-V FITC staining on the y-axis. The percentage of cells in each quadrant are shown in the corners of the quadrant.