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. 2021 Jan 21;11:588669. doi: 10.3389/fphar.2020.588669

FIGURE 4.

FIGURE 4

Dot plot of the percentage of annexin+/PI− human PAECs in each experimental condition with mean ± SEM data superimposed. Cells were incubated in EGM-2 with 10% FBS alone (“growth factor control”), EGM-2 with 2% FBS alone (serum starvation) or EGM-2 with 2% FBS and 18hrs treatment with rhVEGF (10 ng/ml) or CMF-019 (1–10 μM). Growth factor and serum starvation (EBM-2 2% FBS) significantly increased the percentage of annexin+/PI− human PAECs and this could be rescued by 10 ng/ml rhVEGF. CMF-019 did not rescue. Matched ANOVA comparing each condition to the EBM-2 2% FBS condition ***p < 0.001. ## indicates p < 0.01 compared to the “healthy” control (EGM-2 10% FBS).