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. 2021 Feb;12(1):52–68. doi: 10.21037/jgo-20-513

Figure 6.

Figure 6

MIA3 is a shared target of miR-30a and miR-222 in colon cancer cells. (A) Network analysis of mRNA and miRNA was employed for the prediction of the target genes of miR-30a and miR-222 using bioinformatics tools (miRBase, miranda, and TargetScan). (B) Western blot analysis of the effects of miR-30a and miR-222 inhibitor on MIA3 expression in HT-29 and HCT-116 cells with or without hCC-MSC-Exo treatment. *P<0.05 vs. control group; #P<0.05 vs. hCC-MSC-Exo group. (C) Alignment of the miR-30a and miR-222 sequences with that of the 3'-UTR of MIA3. (D) Schematic model of the MIA3 3'-UTR construct with or without mutation at the predicted miR-30a and miR-222 seeding site. (E) Luciferase reporter activity of chimeric vectors containing the luciferase gene and a fragment of the MIA3 3'-UTR containing the wild-type or mutated miR-30a or miR-222 seeding site in HT-29 and HCT-116 cells. *P<0.05 vs. psiCheck2 vector control group.