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. 2021 Feb;12(1):121–132. doi: 10.21037/jgo-21-12

Figure 4.

Figure 4

The combined use of lipoteichoic acid (LTA) and miR-27b-3p agomir has synergistic effect for anti-gastric cancer in vitro. (A) AGS cells and normal gastric mucosa-derived cell line (GES-1) were transfected by increasing concentration of miR-27b-3p agomir (0 nm–70 nmol), and MTT analyzed the cell viability. (B) AGS cells and normal gastric mucosa-derived cell line (GES-1) were transfected by increasing concentration of LTA (0–8 µmol), and cell viability was analyzed by MTT. AGS cells were treated with miR-27b-3p agomir, LTA, or both. The cell viability of AGS cells after indicated treatment was analyzed by MTT (C) and cell apoptosis was analyzed by using Hoechst assays stained with Hoechst (D). All the experiments were carried out in triplicate. *P<0.05, **P<0.01, ***P<0.001.