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The infected adenoviruses were cultured in the supplemented medium for an additional 48 h before evaluation of green fluorescent protein (GFP) expression by FACS analysis. FISH was conducted to verify the successful transfection of adenovirus with miR-155 or vehicle vector into ApoE−/− mouse VSMC after tail vein injection for 3 days. The high ratio of transfection rate guaranteed the subsequent sample analysis.
