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Western blotting and corresponding real-time PCR of carotid artery specimens were performed to investigate the effect of miR-155 on NoxA1-NADPH-p47phox complex and related signaling pathway. miR-155 mimic group showed similar trend as that in in vitro experiments, that was, significant inhibitory effect on NoxA1, Nox1 and p47phox expression while SOD2, the antioxidant was significantly higher than that of inhibitor group.
