Skip to main content
. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biomaterials. 2020 Sep 16;265:120387. doi: 10.1016/j.biomaterials.2020.120387

Figure 3.

Figure 3.

Adipogenic evaluation of SV40LT transduced IPFSCs. IPFSCs transduced with lentivirus carrying GFP served as a viral transduction control (GFP) and those without transduction served as a blank control (CTR). GSEA for expressed genes ranked by the fold change of expression (SV40/GFP) at passage 5 against the MSigDB gene set “BURTON adipogenesis 3”, which includes genes strongly upregulated during differentiation from fibroblasts (3T3) into adipocytes (A). Twenty-one day adipogenically induced IPFSCs from passages 5, 10 and 15 were evaluated using TaqMan® real-time quantitative PCR for adipogenic marker genes (LPL, CEBPA and PPARG) (B). GAPDH served as an internal control gene. Data are exhibited as bar charts. * indicates a statistically significant difference compared to the corresponding GFP control (p<0.05). Adipogenically induced passage 5 IPFSCs were also evaluated using Oil Red O staining for lipid droplets (C) and western blot for LPL expression at the protein level (D).