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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biomaterials. 2020 Sep 16;265:120387. doi: 10.1016/j.biomaterials.2020.120387

Figure 6.

Figure 6.

Chondrogenic evaluation of high-passage IPFSCs after growth on dECMs and TCP. Following expansion on TCP (CC) and dECMs deposited by passage 5 IPFSCs (C5C), passage 15 IPFSCs (C15C), SV40LT transduced passage 5 IPFSCs (S5C) and SV40LT transduced passage 15 IPFSCs (S15C), passage 15 IPFSCs were grown in a pellet culture system (termed CP, C5P, C15P, S5P and S15P, respectively) supplemented with chondrogenic medium. Thirty-day pellets (along with 0-day pellets) were assessed using TaqMan® real-time quantitative PCR for chondrogenic marker genes (SOX9, COL2A1, ACAN, PRG4) and hypertrophic marker gene (COL10A1) (A). GAPDH served as an internal control. Data are exhibited as bar charts. Statistically significant differences are indicated using * when compared to CC, using $ when compared to corresponding C5C or C15C and using # when compared to corresponding C5C or S5C (p<0.05). Histologically, 30-day pellets were evaluated using photographs for the size (mm), Alcian blue staining (Ab) for sulfated GAGs and immunohistochemical staining (IHC) for type II collagen (Col2) (B).