FIG 3.

SHIV.C.CH505 persistence in blood and tissue CD4⁺ T cells. (a and b) Cell-associated SHIV DNA (a) and SHIV RNA (b) in enriched CD4⁺ T or CD11b⁺ cells at the indicated time points. Statistical significance was determined by the Wilcoxon signed-rank test. (c) Log-transformed RNA/DNA ratios in CD4⁺ T cells from the indicated sites after 48 weeks on ART. The Wilcoxon signed-rank test was used for statistical analysis. (d) Replication-competent virus levels in enriched blood and spleen CD4⁺ T cells after ≥50 weeks on ART as determined by QVOA. In all panels, each point represents one animal (n = 6), and the line is at the median. Open symbols indicate values below the LOD. *, P < 0.05; ****, P < 0.0001.