FIG 3.

In vivo replication of vΔUL47 and vΔUL48 in comparison to vRB1B after muscular inoculation. (A) Ten 2-week-old white Leghorn chickens were inoculated with 3,000 PFU of vRB1B, vΔUL47, or vΔUL48. The percentages of surviving animals are shown for vRB1B (WT, red line), vΔUL47 (blue line), and vΔUL48 (green). (B) DNA was extracted from PBMCs of animals inoculated with vΔUL48 and was analyzed by PCR for the presence of the viral genome at 21 dpi. Actin was used as a loading control. Chicken identification numbers with an asterisk indicate animals that survived until the experiment terminated. Of note, one animal in the vΔUL48 group died 2 days postinoculation, possibly of a wound contracted at the foot.