FIG 9.

Expression of gC in CESCs infected with vΔUL47, vΔUL54, vΔgC, and vRB1B. Primary CESCs were infected with vRB1B, vΔUL47 (A), vΔUL54 (B), or a virus that did not express gC (vΔgC). At 6 h before harvesting, the cells were treated or not with 10 μg/ml brefeldin A (BFA) to block the secretion of gC and concentrate it within the cell. Cell lysates were analyzed by Western blot with MAb A6 against gC, MAb H18 against VP5, and an anti-tubulin antibody. Viral protein quantities were normalized to the amount of VP5, and the levels of gC in vΔUL47 and vΔUL54 samples were calculated relative to the levels in the vRB1B sample. Tubulin was used as a loading control.