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. 2021 Feb 17;93(8):3717–3725. doi: 10.1021/acs.analchem.0c03185

Figure 2.

Figure 2

Characterization of EVs from prostate cancer cells with different phenotypes. (a, b) Cell morphologies under bright-phase microscopy. Scale bars are 400 μm. PC3-Epi cells have cuboidal shapes, while PC3-EMT cells are spindle-shaped. (c, d) TEM images confirming the presence of negative-stained EVs, seen as cup-shaped vesicles. Scale bars are 100 nm. (e) Particle concentrations of PC3-Epi and -EMT EV preparations measured by nFCM. The particle concentrations have been normalized using sample input volumes. The error bars represent the standard deviation of triplicated experiments. No significant difference has been found. (f, g) Particle size distributions of PC3-Epi and -EMT EV preparations measured by nFCM. The bin width is 0.5 nm. To make the size distribution histogram visually comparable, the Y axis is adjusted to make the concentration of particles with modal size (the peak of the curve) as 95% of maximum scale in each figure.