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Schematic of the PIXY system. The system consists of a stage-top incubator (A), XY movable stage (B), and four peristaltic pumps (C) equipped with perfusion (D) lines for chemical transfer. Cell culture media is withdrawn from each well. DPBS, hydrogen peroxide, and quench solutions are infused into each well as indicated by the colored arrows. White lines indicate the laser path for irradiation.
