Figure 1.

(a) Differential scanning calorimetry (DSC) traces comparing reversible melting and recrystallization transitions of covalently conjugated and electrostatically conjugated biohybrid variants in the absence of solvent. Comparison of cHEWL:C₁₂EO₁₀ (green dashed line) and HEWL-C₁₂EO₁₀ (red solid line) or cHEWL:C₁₂EO₂₂ (cyan dashed line) and HEWL-C₁₂EO₂₂ (blue solid line). Reduced melting and recrystallization temperatures are observed for covalently conjugated (T_HEWL-C12EO10 = 19 °C; T_HEWL-C12EO22 = 36 °C) biohybrids compared with their charge-stabilized counterparts (T_{cHEWL:C12EO10} = 25 °C; T_{cHEWL:C12EO22} = 43 °C). (b) Representative images of cHEWL:C₁₂EO₂₂ and HEWL-C₁₂EO₂₂ before (25 °C) and after (60 °C) melting.