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. 2020 Sep 10;15(3):471–484. doi: 10.1093/ecco-jcc/jjaa182

Figure 4.

Figure 4.

Tofacitinib reduces STAT signalling in THP-1 macrophages and cytokine IL-6 secretion in co-culture studies. THP-1 macrophages co-cultured with tofacitinib-treated Caco-2BBe cells for 24 h were lysed and subjected to western blotting. Representative western blot images and densitometric analyses of A] phospho-JAK1 [pJAK1], B] phospho-STAT1 [pSTAT1], and C] phospho-STAT3 [pSTAT3] normalised to their respective total forms [tJAK1, tSTAT1, tSTAT3] in THP-1 whole-cell lysates. D] IL-6, IL-22, and IL-10 concentrations present in the culture media after co-culture experiments, measured by ELISA; *p ≤0.05, *** p ≤ 0.001 cf. vehicle-treated Ctr THP-1 without co-culture; §p ≤0.05, §§p ≤0.01, §§§p ≤0.001 cf. vehicle-treated Ctr THP-1 with KD Caco-2BBe; xp ≤0.05, xxp ≤0.01, xxxp ≤0.01 cf. vehicle-treated KD THP-1 without co-culture; ¶p ≤0.05, ¶¶¶p ≤0.001 cf. vehicle-treated KD THP-1 without co-culture; op ≤0.05, ooop ≤0.001 cf. vehicle-treated Ctr THP-1 with Ctr Caco-2BBe; n = 3. The “−” symbol indicates no co-culture.