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. 2021 Mar 7;49(3):0300060519887251. doi: 10.1177/0300060519887251

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Cell proliferation, apoptosis, and expression in NC(+) group, NEAT1(+) group, NC(−) group and NEAT1(−) group. (a) The CCK8 assay showed that the cell proliferation rate was lower in the NEAT1(+) group and higher in the NEAT1(−) group compared with the corresponding NC groups during I/R injury. (b, d) The AV/PI assay showed that cell apoptosis rate was increased in the NEAT1(+) group but decreased in the NEAT1(−) group compared with the corresponding NC groups during I/R injury. (c, e) Western blot assay revealed that C-caspase expression was higher in the NEAT1(+) group relative to the NC(+) group, and Bcl-2 expression was decreased in the NEAT1(+) group but increased in the NEAT1(−) group compared with the corresponding groups during I/R injury. Comparison was determined by t-test. Results are shown as means ± standard deviations. P < 0.05 was considered significant; **P < 0.01; *P < 0.05. CCK-8, Cell Counting Kit-8; AV/PI, annexin V apoptosis detection with propidium iodide; NEAT1, nuclear-enriched abundant transcript 1; NC(+), control overexpression plasmid, NEAT1(+), lnc-NEAT1 overexpression plasmid, NC(−), control short hairpin (sh)RNA plasmid, and NEAT1(−), lnc-NEAT1 shRNA plasmid; I/R, ischemia/reperfusion; C-caspase 3, cleaved caspase 3; Bcl-2, B-cell lymphoma-2.