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. 2021 Feb 21;143(8):3219–3230. doi: 10.1021/jacs.0c12731

Figure 6.

Figure 6

(a). Design of large unilamellar vesicle (LUV) experiments. Before polymer treatment, fluorescence signal from entrapped FITC-labeled dextran is quenched by entrapped TRITC-labeled dextran via FRET. TRITC-labeled dextran (155 kDa) has an average Stokes radius of 85 Å, making it difficult to escape from the pores of liposomes. If polymer treatment causes leakage of vesicle contents, the FITC fluorescence signal will no longer be quenched. (b) LUV dye leakage for four different FITC-labeled dextran sizes caused by TM-containing copolymers at 500 μg/mL. (c) LUV dye leakage caused by TM-containing copolymers at 125 μg/mL. (d) LUV dye leakage caused by DMCH-containing copolymers at 500 μg/mL. (e) LUV dye leakage caused by DMCH-containing copolymers at 125 μg/mL. In these studies, the FITC fluorescence signal is measured immediately after addition of polymer to the LUV preparation.