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. 2021 Feb 24;12:585778. doi: 10.3389/fphar.2021.585778

FIGURE 3.

FIGURE 3

Macrophages display an M1 phenotype in ischemic muscles in response to low doses of cilengitide. (A) Representative images of macrophages as assessed by F4/80 immunostaining in ischemic gastrocnemius muscles in vehicle, cilengitide (50 μg/kg) and cilengitide (5 mg/kg) mice at 14 days after surgery. Scale bars, 100 μm. (B) Quantification of anti-F4/80 positive-macrophage infiltration of ischemic muscles. (C) The gene profile of the M1-and M2-macrophage phenotypes by quantitative RT-PCR of ischemic gastrocnemius muscles 14 days after surgery. All bars show the mean ± SEM (n = 6 per group). *p < 0.05 vs. vehicle; #p < 0.05 vs. cilengitide (50 μg/kg).