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. Author manuscript; available in PMC: 2021 Mar 10.
Published in final edited form as: Mol Pharm. 2019 Dec 16;17(1):98–108. doi: 10.1021/acs.molpharmaceut.9b00788

Figure 2.

Figure 2.

Flow cytometry analysis of the pretargeted click-chemistry delivery. (A) PSMA expression levels in (i) PC3-PIP and (ii) PC3-Flu cells. Combined histograms of untreated cells and cells treated with 5D3(Cy5)2 and F(ab′)2(Cy5)2. (B) Pretargeted delivery in the presence of the click reaction between Alb(DM1)3.3(PEG4-Tz)10(AF-488)2 and reactive (i) 5D3(TCO)8(Cy5)2 in PC3-PIP cells, (ii) F(ab′)2(TCO)8(Cy5)2 in PC3-PIP cells, (iii) 5D3(TCO)8(Cy5)2 in PC3-Flu cells, and (iv) F(ab′)2(TCO)8(Cy5)2 in PC3-Flu cells. (C) Pretargeted delivery in the absence of a click reaction between ALB(DM1)3.3(PEG4-Tz)10(AF-488)2 and unreactive (i) 5D3(Cy5)2 in PC3-PIP cells, (ii) F(ab′)2(Cy5)2 in PC3-PIP cells, (iii) 5D3(Cy5)2 in PC3-Flu cells, and (iv) F(ab′)2(Cy5)2 in PC3-Flu cells.