Table 2.
Mutation bias within T-cell receptor variable (TCRV) segments differed by V segment type
| V segment | Region | % Transition mutations | % Transversion mutations | % All mutations to G or C nucleotides | % G and C mutations inside motifs |
|---|---|---|---|---|---|
| TCRαδV-TCRαC | FR | 45.58 | 43.54 | 59.41 | 53.44 |
| CDR | 43.40 | 43.40 | 51.57 | 70.73 | |
| All | 45.00 | 43.50 | 57.33 | 57.56 | |
| TCRαδV-TCRδC | FR | 53.33 | 34.67 | 62.67 | 55.32 |
| CDR | 52.94 | 47.06 | 64.71 | 54.55 | |
| All | 53.26 | 36.96 | 63.04 | 55.17 | |
| TCRβV | FR | 48.75 | 36.25 | 60.00 | 33.33 |
| CDR | 44.44 | 44.44 | 66.67 | 50.00 | |
| All | 48.31 | 37.08 | 60.67 | 35.19 | |
| TCRγV | FR | 56.00 | 34.67 | 65.33 | 26.53 |
| CDR | 62.50 | 25.00 | 87.50 | 57.14 | |
| All | 56.63 | 33.73 | 67.47 | 30.36 | |
| IgHV-TCRδC | FR | 50.72 | 37.68 | 49.28 | 38.24 |
| CDR | 51.92 | 46.15 | 71.15 | 59.46 | |
| All | 51.05 | 40.00 | 55.26 | 45.71 | |
| NTCRV | FR | 42.86 | 47.62 | 42.86 | 30.00 |
| CDR | 62.50 | 25.00 | 37.50 | 27.27 | |
| All | 48.28 | 41.38 | 41.38 | 28.57 | |
| STCRδV | FR | 40.91 | 54.55 | 45.45 | 20.00 |
| CDR | 66.67 | 33.33 | 33.33 | 0.00 | |
| All | 44.00 | 52.00 | 44.00 | 18.18 |
FR, framework region; CDR, complementarity-determining region; TCRαδV-TCRαC, alpha; TCRαδV-TCRδC, delta; TCRβV, beta; TCRγV, gamma; IgHV-TCRδC, trans-rearrangements between immunoglobulin heavy chain (IgH) and TCRδ constant regions; NTCRV and STCRδV, distal and proximal (supporting) components of NAR-TCR.