Table 1.
Primers used in this study
| Target genes | Primer sequence (5′–3′) | Amplicon (bp) | References |
|---|---|---|---|
| PCR and sequencing | |||
| OXA-51 |
F: TAATGCTTTGATCGGCCTTG R: TGGATTGCACTTCATCTTGG |
353 | [23] |
| rpoB |
F: GTGATAARATGGCBGGTCGT R: CGBGCRTGCATYTTGTCRT |
543 | [24] |
| adeB |
F: TTAACGATAGCGTTGTAACC R: TGAGCAGACAATGGAATAGT |
541 | [30] |
| adeG |
F: TTCATCTAGCCAAGCAGAAG R: ATGTGGGCTAGCTAACGGC |
652 | [9] |
| adeJ |
F: ATTGCACCACCAACCGTAAC R: TAGCTGGATCAAGCCAGATA |
463 | [30] |
| adeR |
F: ATGAGTGTGTAGGGATAATC R: TACTACAGAAAATAGCGTAAC |
961 | This study |
| adeSa |
F: ATGTTAATTAATGTGCGTGGC R: CAGCTTATATGTTAGGTGTCC |
1315 | This study |
| adeS |
F: ATGAAAAGTAAGTTAGGAATTAGTAAG R: TTAGTTATTCATAGAAATTTTTATG |
1074 | [15] |
| baeS1b |
F1: TAAAGATGAAGAAACAATGGAG R1: ATCACCTAACTCATCATTTCG |
1024 | This study |
| baeS2b |
F2: CCAGTTTTAGATAAAGAAGATGC R2: ACCAGATGCGCTAACTCGAC |
1014 | This study |
| baeR |
F: CATTAGGTGGTTTACGTTGTG R: CTGATGTCGCGGTGAGCTG |
845 | This study |
| adeL |
F: TGGACGGAGCATAAAAGTTTG R: CAATTTCTATACCATAAGTTAAGG |
1246 | This study |
| adeN |
F: AAACATACCAATGACCATCG R: GTAGCTACTCCATAATAATTG |
842 | This study |
| RT-PCR analysis | |||
| adeB |
F: AACGGACGACCATCTTTGAGTATT R: CAGTTGTTCCATTTCACGCATT |
84 | [15] |
| adeG |
F: AACTATGCGGTGCTCAAC R: GGTGAATTACTTGGTGATGC |
218 | [5] |
| adeJ |
F: AGCTGGTGCTATGGGCGTTA R: GCCACCCCATGCAATACG |
64 | [31] |
| rpoB |
F: GAGTCTAATGGCGGTGGTTC R: ATTGCTTCATCTGCTGGTTG |
110 | [14] |
aPrimer sets designed in this study for adeS gene, but could not successfully amplify it
bSince the complete sequence of the baeS gene is 1659 bp, we designed two overlapping primer pairs for full-length amplification and sequencing of this gene