Fig. 5.

High dose of AAV8-Hmox1 virus causes mislocalization of rhodopsin and opsin. a, b Immunostaining of rhodopsin in the retinas from albino mice 2 weeks after infection with a low or high dose of AAV8-GFP (a) (n = 3) or AAV8-HMOX1 (b) (n = 3). Note that rhodopsin was abnormally present in the ONL only in the retinas infected with the high dose of AAV8-HMOX1 (arrows). c, d Immunostaining of opsin in the retinas from the albino mice 2 weeks after infection with a low or high dose of AAV8-GFP (n = 3) or AAV8-HMOX1 (n = 3). Note that Opsin was mislocalized in the ONL of the retinas infected with the high dose of AAV8-HMOX1 (arrows). ONL, outer nuclear layer; IS, photoreceptor inner segments; OS, photoreceptor outer segments. Scale bars: 20 μm