Figure 2.

BBV152 vaccine induces high virus-specific antibody response in mice

(A–I) Immune response elicited against antigens at three concentrations of antigen or adjuvanted vaccine formulations in BALB/c mice (n = 5, female) were represented. Animals were administered via IP route either with 1/20^th (Fig A & B) or 1/10^th (Fig D, E, & F) human single dose (HSD) or administered via intramuscular route with either full HSD or 1/10th dose (C, G, & H). S1-specific total IgG antibody binding titers measured using individual sera collected (A) at various time points (day 0, 7, 14, & 21) at 1/20^th dose; (D) on day 21 with 1/10^th dose; (C) on day 21 either with full HSD or 1/10^th dose; (G)on post-dose 1 (on day 7 or 14) and 2 (on day 14 or 28), when BALB/c mice were administered with BBV152B via IM route with different immunization schedule with an interval of 7 or 14 days, and (H) at various time points (day 21, 28, 42, 56, 84, and 98) at 1/10^th dose via IM route. (E) SARS-CoV-2 specific (S1, RBD, N and total inactivated antigen) antibody binding titers elicited against adjuvant vaccines (BBV152A, B & C) on day 21; neutralizing antibody titers performed by PRNT_90, using day 21 sera collected from BALB/c mice, when administered via IP route either with 1/20^th (B) or 1/10^th dose (F) or collected at various time points day 21, 28, 42, 56, 84, and 98 (I), when administered at 1/10^th dose via IM route. Antibody binding titers were performed by ELISA and neutralizing antibody titers by PRNT₉₀. Bar graphs representing data represented as mean ± SD (G), mean/mean ± SEM (A, H, & I) derived from individual mice sera data analysis. For the long-term immunogenicity study, sera from four animals per group were tested for ELISA and MNT₅₀ analyzed. Statistical analysis performed by (B) Wilcoxon rank test indicates significant difference between 0.3 μg antigen Algel and 0.3 μg antigen Algel-IMDG at p value < 0.05 and error bars indicate median with 95% CI, whereas in figure D, statistical analysis performed by Mann Whitney test showed significant difference at p value < 0.005 (∗∗) and <0.05 (∗), respectively. ns indicates not significant.