Fig. 2.

High content screening assay for SARS-CoV2. A) Scheme of SARS-CoV2 HCA. Huh7-hACE2 were seeded onto 96-well plates, after 24 h cells were treated with the drugs in two-fold dilutions and immediately infected with SARS-CoV2 (MOI 0.1). 20 h after infection, cells were fixed, stained and analyzed. B) Representative images of the HCA with the control drug hydroxychloroquine (HCQ) (Wang et al., 2020) (Liu et al., 2020). Nuclei are stained by DAPI (red) and Spike (S) is stained with the mSPI-3022 antibody (green), scale bar corresponds to 50 μm. C) Dose response of the positive control hydroxychloroquine. White dots represent the percentage of normalized % of inhibition. Blue triangles represent the % of nuclei compared to the average % of non-infected cells. Error bars represent the standard deviation (SD) of 2 independent experiments.