Fig. 3.

MiR-325-3p directly targets S100A4. a The complementary sequences of miR-325-3p were identified in 3′UTR of S100A4 mRNA using TargetScan. b miR-325-3p inversely modulated the luciferase activity of plasmids containing WT 3′UTR of S100A4 (WT, Wild Type; Mut, Mutant Type) (mean ± SD). c The protein level of S100A4 in OCPs transfected with agomir-325-3p, antagomir-325-3p or their controls were tested by Western blot. d The relative intensity of S100A4 comparing with GAPDH in (c) was quantified (mean ± SE). In vitro experiments were performed at least 3 times. *p < 0.05, **p < 0.01, ***p < 0.001