Fig. 5.

MiR-325-3p treatment attenuates bone resorption in bone metastasis of CRC. a The agomir-325-3p or antagomir-325-3p or their controls were injected through lateral veins weekly for three weeks after injection of CT-26 cells intratibially. The samples were collected and sectioned. Safranin O-Fast Green staining revealed trabecular area in each group (Scar bar = 50 μm) (n = 3–5 mice in each group). b Quantification of trabecular area in (a) (mean ± SE). c TRAP staining revealed TRAP positive cells in trabecular area (Scar bar = 50 μm) (n = 3–5 mice in each group). d Quantification of osteoclast surface comparing with trabecular surface in (c) (mean ± SE). In vitro experiments were performed at least 3 times.*p < 0.05, **p < 0.01, ***p < 0.001