Fig. 4.

Phylogenetic tree of the Stomoxys calcitrans IRs with those of Drosophila melanogaster and Musca domestica. Maximum likelihood tree rooted with the Ir8a/25a lineage as the outgroup. The S. calcitrans and M. domestica lineages are in teal and blue, respectively, while D. melanogaster lineages are in mustard. Support levels from the approximate likelihood-ratio test from the PhyML v3.0 are shown. Subfamilies, clades, and individual Drosophila genes are indicated outside the circle to facilitate finding them in the tree. Pseudogenic sequences are indicated with the suffix P. Scale bar indicates amino acid substitutions per site. The full phylogenetic tree can be found in Additional file 1: Figure S12