Fig. 6.

Maximum likelihood phylogenetic analysis of yolk protein genes from Drosophila melanogaster, Glossina morsitans, Musca domestica, and Stomoxys calcitrans. Gene sequences with significant homology were aligned using the ClustalO software package [221], and the alignment used to estimate a Maximum likelihood phylogeny in CLC Main Workbench (construction method: neighbor joining, Protein substitution model: WAG, Bootstrap analysis: 1000 replicates). Numerical annotations indicate bootstrap values for each branch point in the tree. Heat map of gene expression (transcripts per million, TPM) is based on RNA-Seq data (see Additional file 4). RS, reproductive system