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. 2021 Mar 10;12:170. doi: 10.1186/s13287-021-02206-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Accumulation of MDSCs in DM and promotion of high glucose on MDSCs. a–c The accumulation of MDSCs was showed by flow cytometry in bone marrow, PBMC and spleen of DM and CON at day 3 after injury. d–f The accumulation of MDSCs in bone marrow, PBMC, and spleen at day 7 after injury was showed by flow cytometry. g, h MDSCs of skin tissue around the wound at day 3 and day 7 after injury were investigated by immunofluorescence staining. i The percentage of MDSCs was analyzed by flow cytometry in bone marrow cells from CM treated with the graded concentration of glucose in vitro. MDSCs, myeloid-derived suppressor cells; BM, bone marrow; CON, control mice; DM, diabetic mice; PBMC, peripheral blood mononuclear cell