Fig. 4.

The expressions of the 4EBP1 and S6 along with p-4EBP1 and p-S6 proteins in MDSCs. a The expression of 4EBP1, S6, P-4EBP1, and P-S6 was analyzed by Western blot in MDSCs from spleen of CON and DM. b The expression of 4EBP1, S6, P-4EBP1, and P-S6 was analyzed by Western blot in MDSCs from bone marrow under presence of 5 mM and 30 mM glucoses, respectively. MDSCs, myeloid-derived suppressor cells; CON, control mice; DM, diabetic mice; P-4EBP1, phosphorylated mammalian target of rapamycin; P-S6, phosphorylated ribosomal protein S6. The experiments were tripled. β-Tublin was used as the reference