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. 2021 Mar 5;14:689–710. doi: 10.2147/JIR.S299083

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© 2021 Sucedaram et al.

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Immunohistochemical staining of IL-6 (A), TNF-α (B) and MCP-1 in liver (C) and quantification of mean pixel intensities of hepatic IL-6 (D), TNF-α (E) and MCP-1 (F) staining of normal diet (ND)- and high fat style diet (HFSD)-fed rats, with and without ovariectomy (OVX) (n = 12 per group) at 40X magnification. Dark red arrow indicates the positive immunohistochemical stainings. **P < 0.01 or ****P < 0.001 vs. ND; ^##P < 0.01 or ^####P < 0.001 vs. OVX-ND; ^δP < 0.05 vs. HFSD.